Zobell Marine Broth contains the nutrients, which are required for the growth of marine bacteria. These media have minerals as in seawater (7) and peptic digest of animal tissue and yeast extract as the sources of nutrients for the marine bacteria as reported by Jones (8). High amount of salt content is used to simulate seawater. Other minerals are used to mimic the mineral composition of seawater.

Directions: Suspend 40.25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Zobell Marine Broth contains the nutrients, which are required for the growth of marine bacteria. These media have minerals as in seawater (7) and peptic digest of animal tissue and yeast extract as the sources of nutrients for the marine bacteria as reported by Jones (8). High amount of salt content is used to simulate seawater. Other minerals are used to mimic the mineral composition of seawater.

Directions: Suspend 40.25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.